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An ELISA kit for the detection of CD26 Human This uses Sandwich ELISA Double Antibody and has a sensitivity of 0 188ng ml
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The Human CD26 (DPP4) ELISA research-use-only kit is an enzyme-linked immunosorbent assay designed for the quantitation and detection of CD26 (DPP4) in samples (see sample types indicated) using a microplate reader. The assay will recognize
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The Human DPPIV CD26 ELISA Kit Colorimetric from Novus Biologicals is a ELISA Kit Colorimetric that quantifies human DPPIV CD26 in human cell culture supernatant serum
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Human CD26/DPP4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD26/DPP4 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium
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Human CD26/DPP4 AccuSignal ELISA Kit - KOA0401
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Image Search Results
Journal: Cells
Article Title: Avian Reovirus P17 Suppresses Angiogenesis by Promoting DPP4 Secretion
doi: 10.3390/cells10020259
Figure Lengend Snippet: ARV p17-mediated release of Dipeptidyl Peptidase 4 (DPP4) expression. ( A ) Wound healing assay performed after HUVECs’ overnight stimulation with conditioned medium from Mock- or ARV p17-nucleofected HUVEC cells. Confluent cell monolayers were scratched using a 200 μL pipette tip and cell migration was recorded by light microscopy 10 h after wound scratch (original magnification, 4×). The wound width was measured, and the relative wound area was calculated as the ratio of the remaining area at the 10 h time point to the 0 h starting point. Pictures are representative of one out of two independent experiments with similar results. Values are the mean ± SD of one representative experiment out of two with similar results, performed in triplicates. Statistical analysis was performed by Student’s t test. *** p < 0.001. ( B ) Tube formation assay performed with HUVECs co-cultivated for 48 h with Mock- or ARV p17-nucleofected HUVEC cells. The pictures were taken 6 h after cell seeding (original magnification, 4×). Closed rings were counted as a parameter for quantification of tube formation. Pictures are representative of one out of two independent experiments with similar results. Values are the mean ± SD of one representative experiment out of two with similar results, performed in triplicates. Statistical analysis was performed by Student’s t test. *** p < 0.001. ( C ) Angiogenesis array performed with the supernatants of Mock- or ARV p17-nucleofected cells recovered 24 h post-nucleofection. Relative pixel intensity was calculated using ImageJ software and expressed as the mean of duplicate dots. Values are representative of one experiment out of two with similar results. ( D ) Analysis of DPP4 gene expression performed using quantitative real-time PCR in Mock- and ARV p17-nucleofected cells. Analysis of real-time PCR data were performed with the 2 -DDCt method using relative quantitation study software. Quantification of DPP4 mRNA was normalized according to the internal β-actin control. Values represent the mean ± SD of one representative experiment out of two with similar results, performed in triplicates. Statistical analysis was performed by Student’s t test. *** p < 0.001. ( E ) soluble (s) DPP4 levels in supernatants of HUVECs stimulated with GST-ARV p17 for 48 h were quantified with human sDPP4 ELISA. Supernatant from GST stimulated cells was used as a negative control. Bar graph displays the concentration of DPP4 determined by absorbance quantification. Values are the mean ± SD of one representative experiment out of three independent experiments with similar results, performed in triplicates. Statistical analysis was performed by Student’s t test. *** p < 0.001.
Article Snippet: The release of DPP4 in the concentrated conditioned medium was evaluated using a
Techniques: Expressing, Wound Healing Assay, Transferring, Migration, Light Microscopy, Tube Formation Assay, Software, Gene Expression, Real-time Polymerase Chain Reaction, Quantitation Assay, Control, Enzyme-linked Immunosorbent Assay, Negative Control, Concentration Assay